DAx has all the features that are needed for chromatographic applications in
general, such as the ability to measure sequences of measurements, recognise and
quantify components, and compare measurements.|
It also contains a number of features geared specifically towards Size Exclusion Chromatography / Gel Permeation Chromatography:
New application note:
This application note also serves as a manual on the interpretation of
molecular weight distribution data in text files.
Calibration curves can be multi-linear, cubic spline, or polynomial (up to 10th order).
Curves can be plotted linearly or logarithmically.
|Calibration Curves relating Molecular Weight to Elution Volume|
Peak lists can display one or all of
- Mp (mol weight at peak top)
- Mn (number averaged mol weight)
- Mw (weight averaged mol weight)
- D (dispersivity)
- Mol weight at peak start / Mol weight at peak end
Traditionally, chromatographic measurements have a horizontal axis depicting
time coordinates. Using an Mw calibration, DAx can convert measurements
to have a horizontal axis depicting molecular weight or log(Mw).
Chromatographic measurements typically measure component concentration or
component weight. The distinction is important when calculating peak parameters
and when drawing molecular weight distributions.
|Converting between concentration and weight data|
Components can be identified by Mp, Mn, Mw or Mz.
|Identifying components by molecular weight|
Data can be plotted with a molecular weight axis instead of a time axis. If baselines
are present, they can be automatically subtracted from the data to give a much cleaner plot.
The plots can be normalised so that the accumulative molecular weight in all peaks that
are present is 100%, making it very easy to compare molecular weight distributions
between different measurements.
|Molecular weight axes|
When the mouse cursor is tracked over a graph, the molecular weight at the cursor's coordinate